Crusaders Of The Lost Idols

Crusaders of the lost idols is an idle game whose creators have shown themselves to be trans friendly.

Alacrity the Matchmaker
Alacrity despite being nonhuman is clearly genderfluid changing with the number of magical vs non-magical crusaders around her.

Langley the Conductor
Langley is simply a nonbinary human crusader. There's no other effect other than the nonbinary tag.

Koivu the Dryad
Koivu is mentioned to have transitioned to male in his introduction description.

other non-binary and genderqueer crusaders
some other crusaders due to being non human can't be considered trans but are non-binary

Plant crusaders
most crusaders with the plant tag also have both the male and female tags referencing plants often have both male and female parts

The Evanescent
The Evanescent doesn't have any gender tags themselves but will most likely gain one or more from their "The Great Equalizer" ability which will also grant those tags to any other crusaders in the same column. Yes, this crusader can make any other crusader trans.

Arenvarr the Beheaded, Drosenthes and Xygallix
Arenvarr the Beheaded, Drosenthes and Xygallix all have the non-binary tag. But since they are not human it is uncertain if they are trans.